3SPH
Inward rectifier potassium channel Kir2.2 I223L mutant in complex with PIP2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-27 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | I 4 |
| Unit cell lengths | 82.725, 82.725, 185.192 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.303 - 3.003 |
| R-factor | 0.2186 |
| Rwork | 0.217 |
| R-free | 0.25370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3spg |
| RMSD bond length | 0.012 |
| RMSD bond angle | 0.977 |
| Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.100 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.097 | 0.600 |
| Number of reflections | 12263 | |
| <I/σ(I)> | 27.4 | 2.7 |
| Completeness [%] | 98.0 | 89.9 |
| Redundancy | 6.3 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 12.1% PEG400, 0.5 M potassium chloride, 0.05 M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
