3SNP
Crystal structure analysis of iron regulatory protein 1 in complex with ferritin H IRE RNA
Replaces: 2IPYExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 78 |
| Detector technology | CCD |
| Collection date | 2005-08-17 |
| Detector | MARMOSAIC 300 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 109.555, 80.851, 142.873 |
| Unit cell angles | 90.00, 92.03, 90.00 |
Refinement procedure
| Resolution | 99.000 - 2.800 |
| R-factor | 0.199 |
| Rwork | 0.198 |
| R-free | 0.21800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 2B3X AND 2B3Y |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 2.910 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.112 | 0.359 |
| Number of reflections | 61046 | |
| <I/σ(I)> | 7.6 | 3.1 |
| Completeness [%] | 97.9 | 91.1 |
| Redundancy | 2.7 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.06 | 291 | 220 mM ammonium phosphate monobasic, 50 mM sodium acetate, pH 5.06, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
