3SLS
Crystal Structure of human MEK-1 kinase in complex with UCB1353770 and AMPPNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.2 |
| Synchrotron site | SRS |
| Beamline | PX14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | CUSTOM-MADE |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.502, 153.890, 48.209 |
| Unit cell angles | 90.00, 101.48, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.300 |
| R-factor | 0.20975 |
| Rwork | 0.206 |
| R-free | 0.28654 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.287 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.290 |
| High resolution limit [Å] | 2.300 |
| Number of reflections | 30851 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 18% PEG-3350, 200mM Ammonium fluoride, 4% Glycerol, 10mM DTT, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
