3SIQ
Crystal Structure of autoinhibited dIAP1-BIR1 domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-07-16 |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | P 31 |
Unit cell lengths | 99.782, 99.782, 71.344 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 32.980 - 2.400 |
R-factor | 0.14959 |
Rwork | 0.146 |
R-free | 0.21709 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1sdz |
RMSD bond length | 0.005 |
RMSD bond angle | 0.990 |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.400 |
Number of reflections | 30880 |
Completeness [%] | 99.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 291 | 100mM Bis-Tris pH 5.4, 0.2M (NH4)2SO4, 3%( vol/vol) CH3OH, 22% (wt/vol) Polyethylene Glycol 3350 , VAPOR DIFFUSION, HANGING DROP, temperature 291K |