3SFG

crystal structure of murine norovirus RNA dependent RNA polymerase in complex with 2thiouridine(2TU)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 4A
Synchrotron site	PAL/PLS
Beamline	4A
Temperature [K]	277
Detector technology	CCD
Collection date	2010-10-10
Detector	ADSC QUANTUM 315
Wavelength(s)	1
Spacegroup name	C 1 2 1
Unit cell lengths	120.794, 195.972, 109.335
Unit cell angles	90.00, 114.86, 90.00

Refinement procedure

Resolution	31.621 - 2.209
R-factor	0.2034
Rwork	0.203
R-free	0.25260
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.223
Refinement software	PHENIX (1.7.1_743)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.200
Rmerge	0.111
Number of reflections	113874
<I/σ(I)>	12.5
Completeness [%]	99.4
Redundancy	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	2.0M ammonium sulphate, cacodylate pH 6.5, 0.2M sodium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 277K