3SE4

human IFNw-IFNAR ternary complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2010-02-05
Detector	ADSC QUANTUM 315
Spacegroup name	P 61 2 2
Unit cell lengths	93.490, 93.490, 403.130
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.845 - 3.500
Rwork	0.213
R-free	0.28800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3s98 3s9d
RMSD bond length	0.014
RMSD bond angle	1.799
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.845	3.600
High resolution limit [Å]	3.500	3.500
Number of reflections	13999
<I/σ(I)>	10.8	2
Completeness [%]	99.1	99.7
Redundancy	9.5	8.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5	293	19% (w/v) PEG 3350, 100 mM Ammonium sulfate, 100 mM Bis-Tris pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K