3SDI
Structure of yeast 20S open-gate proteasome with Compound 20
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Detector technology | CCD |
Detector | MAR CCD 165 mm |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 136.600, 299.450, 145.483 |
Unit cell angles | 90.00, 113.17, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.650 |
R-factor | 0.2193 |
Rwork | 0.219 |
R-free | 0.25320 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1g0u |
RMSD bond length | 0.010 |
RMSD bond angle | 1.258 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 2.740 |
High resolution limit [Å] | 2.650 | 5.700 | 2.650 |
Rmerge | 0.099 | 0.050 | 0.378 |
Number of reflections | 297624 | ||
<I/σ(I)> | 9.5 | ||
Completeness [%] | 95.8 | 99.5 | 78.8 |
Redundancy | 3.7 | 3.8 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 100 mM MES, pH 7.0, 40 mM MgOAc, 15% 2-methyl-2,4-pentanediol (MPD), 10 mM EDTA, vapor diffusion, hanging drop, temperature 293K |