3SBB
Disulphide-mediated Tetramer of T4 Lysozyme R76C/R80C by Synthetic Symmetrization
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-07-24 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9795 |
Spacegroup name | P 4 2 2 |
Unit cell lengths | 83.988, 83.988, 58.923 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.236 - 1.434 |
R-factor | 0.141 |
Rwork | 0.139 |
R-free | 0.17750 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.222 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 80.000 | 80.000 | 1.480 |
High resolution limit [Å] | 1.430 | 3.080 | 1.430 |
Rmerge | 0.063 | 0.035 | 0.534 |
Number of reflections | 38885 | ||
<I/σ(I)> | 14.5 | ||
Completeness [%] | 99.3 | 93.9 | 100 |
Redundancy | 5.4 | 5.2 | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 290 | 4.3M Sodium Chloride, 0.1M HEPES, pH 7.5, vapor diffusion, hanging drop, temperature 290K |