3SBA
Zn-mediated Hexamer of T4 Lysozyme R76H/R80H by Synthetic Symmetrization
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-07-24 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97949 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 55.470, 137.780, 146.940 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.765 - 2.750 |
R-factor | 0.2248 |
Rwork | 0.222 |
R-free | 0.27790 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.601 |
Data scaling software | XSCALE |
Phasing software | PHASER (2.1.4) |
Refinement software | PHENIX (1.7_650) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 19.765 | 2.820 | |
High resolution limit [Å] | 2.750 | 12.300 | 2.750 |
Rmerge | 0.219 | 0.179 | 0.490 |
Number of reflections | 29680 | 473 | 3619 |
<I/σ(I)> | 5.31 | 9.25 | 1.88 |
Completeness [%] | 97.1 | 75.1 | 87.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 290 | 0.17M Ammonium Acetate, 0.085 Sodium Acetate Trihydrate, 25.5% PEG 4000, 15% Glycerol, pH 4.6, vapor diffusion, hanging drop, temperature 290K |