3SB5

Zn-mediated Trimer of T4 Lysozyme R125C/E128C by Synthetic Symmetrization

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2010-07-24
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97949
Spacegroup name	H 3 2
Unit cell lengths	128.970, 128.970, 295.140
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.500 - 2.460
R-factor	0.1868
Rwork	0.184
R-free	0.23040
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.090
Data scaling software	SCALEPACK
Phasing software	PHASER (2.1.4)
Refinement software	BUSTER-TNT (BUSTER 2.8.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	80.000	80.000	2.540
High resolution limit [Å]	2.450	5.280	2.450
Rmerge	0.108	0.079	0.508
Number of reflections	34697
<I/σ(I)>	10.1
Completeness [%]	99.9	99.3	99.9
Redundancy	8.7	8.7	8.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	290	0.2M Magnesium Chloride, 20% PEG 8000, 0.1M TRIS, pH 8.5, vapor diffusion, hanging drop, temperature 290K