3S9L
Complex between transferrin receptor 1 and transferrin with iron in the N-Lobe, cryocooled 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-06-08 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9795 |
Spacegroup name | P 43 2 2 |
Unit cell lengths | 231.676, 231.676, 168.955 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 3.220 |
Rwork | 0.271 |
R-free | 0.31430 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER (2.1.4) |
Refinement software | CNS |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 3.330 |
High resolution limit [Å] | 3.220 | 6.910 | 3.220 |
Rmerge | 0.142 | 0.040 | 0.991 |
Number of reflections | 74056 | ||
<I/σ(I)> | 8.1 | ||
Completeness [%] | 100.0 | 99.9 | 99.7 |
Redundancy | 30.6 | 31.9 | 20.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 5% PEG 3350, 0.2 M MgCl2, 10% 1,2-propanediol, pH Hepes 7.5, vapor diffusion, hanging drop, temperature 293K |