3S94

Crystal structure of LRP6-E1E2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2010-12-05
Detector	ADSC QUANTUM 315
Spacegroup name	P 1 21 1
Unit cell lengths	70.540, 144.510, 70.760
Unit cell angles	90.00, 96.00, 90.00

Refinement procedure

Resolution	30.000 - 2.800
R-factor	0.24008
Rwork	0.237
R-free	0.29611
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ijq
RMSD bond length	0.007
RMSD bond angle	1.276
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.800
Number of reflections	34730
Completeness [%]	100.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	298	80mM sodium citrate pH 5.5, 20-21% PEG3350, 40mM KSCN, VAPOR DIFFUSION, HANGING DROP, temperature 298K