3S8P

Crystal Structure of the SET Domain of Human Histone-Lysine N-Methyltransferase SUV420H1 In Complex With S-Adenosyl-L-Methionine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-05-11
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97904
Spacegroup name	P 1
Unit cell lengths	46.424, 50.134, 74.839
Unit cell angles	100.99, 108.05, 89.76

Refinement procedure

Resolution	33.940 - 1.850
R-factor	0.1882
Rwork	0.187
R-free	0.21160
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.241
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	REFMAC (refmac_5.6.0081)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.890
High resolution limit [Å]	1.850	4.560	1.850
Rmerge	0.068	0.058	0.509
Number of reflections	52506
<I/σ(I)>	17.7
Completeness [%]	97.8	99.3	96.8
Redundancy	3.9	3.9	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	295	2.0 M sodium formate, 0.1 M BisTris propane, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K