3S88
Crystal structure of Sudan Ebolavirus Glycoprotein (strain Gulu) bound to 16F6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2009-04-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9793 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 193.591, 193.591, 193.591 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.630 - 3.351 |
| R-factor | 0.22173 |
| Rwork | 0.222 |
| R-free | 0.27759 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3csy |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.426 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.430 |
| High resolution limit [Å] | 3.350 | 3.350 |
| Number of reflections | 17047 | |
| <I/σ(I)> | 10.1 | 1.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.3 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.4 | 298 | 15% PEG 3350, 0.2 M Lithium citrate, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
