3S6B

Crystal structure of methionine aminopeptidase 1b from Plasmodium Falciparum, PF10_0150

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2011-05-10
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	58.868, 52.581, 63.506
Unit cell angles	90.00, 116.67, 90.00

Refinement procedure

Resolution	35.000 - 1.950
R-factor	0.1803
Rwork	0.178
R-free	0.22710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2b3h
RMSD bond length	0.014
RMSD bond angle	1.413
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	1.930
High resolution limit [Å]	1.900	5.150	1.900
Rmerge	0.069	0.033	0.637
Number of reflections	27052
<I/σ(I)>	11.7		1.81
Completeness [%]	98.1	99.7	78.8
Redundancy	3.5	3.6	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	27% PEG3350, 0.2 M Ammonium Acetate, 0.1 M Tris, 2 mM TCEP, 20 % glycerol, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K