3S62
Structure of Fab fragment of malaria transmission blocking antibody 2A8 against P. vivax P25 protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-03-14 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.0 |
Spacegroup name | C 2 2 2 |
Unit cell lengths | 134.075, 177.819, 74.624 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 4.010 |
R-factor | 0.26646 |
Rwork | 0.264 |
R-free | 0.31001 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1z3g |
RMSD bond length | 0.013 |
RMSD bond angle | 1.663 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | |
High resolution limit [Å] | 4.000 | 4.009 |
Rmerge | 0.151 | |
Number of reflections | 7444 | |
<I/σ(I)> | 13.2 | 13.2 |
Completeness [%] | 99.7 | 99.7 |
Redundancy | 7.3 | 13.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 0.1 M MES, 25% PEGMME550, 10mM ZnSO4, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |