3S0X
The crystal structure of GxGD membrane protease FlaK
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-02-12 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 70.895, 99.715, 118.436 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.560 - 3.600 |
R-factor | 0.27602 |
Rwork | 0.270 |
R-free | 0.32713 |
Structure solution method | SAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.611 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 3.730 |
High resolution limit [Å] | 3.600 | 3.600 |
Number of reflections | 10106 | |
Completeness [%] | 99.0 | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 9.5 | 298 | 30% PEG300 50 mM glycine 100 mM NaCl, pH 9.5, EVAPORATION, temperature 298K |