3RZ3
Human Cdc34 E2 in complex with CC0651 inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-27 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97918 |
| Spacegroup name | F 2 2 2 |
| Unit cell lengths | 121.797, 139.525, 216.059 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.300 |
| R-factor | 0.22278 |
| Rwork | 0.221 |
| R-free | 0.25821 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ob4 |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.081 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 25.000 | 30.000 | 2.280 |
| High resolution limit [Å] | 2.300 | 4.730 | 2.200 |
| Number of reflections | 32630 | ||
| Completeness [%] | 87.4 | 84.4 | 47.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.5M NaH2PO4, 1.6M K2HPO4, 0.1 M Imidazole, 0.2 M NaCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
