3RYT

The Plexin A1 intracellular region in complex with Rac1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2008-12-07
Detector	ADSC QUANTUM 315
Spacegroup name	P 43 21 2
Unit cell lengths	110.783, 110.783, 265.628
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.544 - 3.582
R-factor	0.2703
Rwork	0.270
R-free	0.33970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 3IG3 AND 1MH1
RMSD bond length	0.004
RMSD bond angle	0.494
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.6.4_486))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.568	3.730
High resolution limit [Å]	3.582	3.600
Number of reflections	20090
Completeness [%]	99.6	98.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	15% PEG1500, 100 mM SPG buffer, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K