3RYO

Crystal Structure of Enhanced Intracellular Survival (Eis) Protein from Mycobacterium tuberculosis with Acetyl CoA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	CCD
Collection date	2009-03-11
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	108.129, 150.190, 184.383
Unit cell angles	90.00, 103.05, 90.00

Refinement procedure

Resolution	29.937 - 2.800
R-factor	0.1946
Rwork	0.192
R-free	0.24770
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.230
Refinement software	PHENIX (1.6.4_486)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.800
Number of reflections	269936

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	12%(w/v) PEG 3350, 300mM ammonium citrate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K