3RY9
Crystal Structure of the Resurrected Ancestral Glucocorticoid Receptor 1 in complex with DOC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-08 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 139.421, 49.144, 100.369 |
| Unit cell angles | 90.00, 105.82, 90.00 |
Refinement procedure
| Resolution | 96.670 - 1.950 |
| Rwork | 0.181 |
| R-free | 0.21607 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2q3y |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.093 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 96.674 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Number of reflections | 48012 | |
| <I/σ(I)> | 13 | |
| Completeness [%] | 93.9 | 78.3 |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 273 | 2.5-2.8 M sodium acetate trihydrate, 0.1 M Bis-Tris propane, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 273K |
