3RWR
Crystal structure of the human XRCC4-XLF complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-24 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.2536 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 745.379, 149.585, 80.475 |
| Unit cell angles | 90.00, 94.72, 90.00 |
Refinement procedure
| Resolution | 49.027 - 3.943 |
| R-factor | 0.2735 |
| Rwork | 0.271 |
| R-free | 0.32560 |
| Structure solution method | MR-SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.788 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.200 |
| High resolution limit [Å] | 3.900 | 3.900 |
| Number of reflections | 78630 | |
| Completeness [%] | 97.6 | 98.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 1.8M Ammonium citrate, pH 8.0, 20 mM Barium chloride dihydrate, 400 mM sodium thiocyanate, 0.5 mM tantalum bromide, 60% PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
