3RWR
Crystal structure of the human XRCC4-XLF complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-11-24 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.2536 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 745.379, 149.585, 80.475 |
Unit cell angles | 90.00, 94.72, 90.00 |
Refinement procedure
Resolution | 49.027 - 3.943 |
R-factor | 0.2735 |
Rwork | 0.271 |
R-free | 0.32560 |
Structure solution method | MR-SAD |
RMSD bond length | 0.003 |
RMSD bond angle | 0.788 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 4.200 |
High resolution limit [Å] | 3.900 | 3.900 |
Number of reflections | 78630 | |
Completeness [%] | 97.6 | 98.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 1.8M Ammonium citrate, pH 8.0, 20 mM Barium chloride dihydrate, 400 mM sodium thiocyanate, 0.5 mM tantalum bromide, 60% PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 277K |