3RW9

Crystal Structure of human Spermidine Synthase in Complex with decarboxylated S-adenosylhomocysteine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2007-06-09
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97918
Spacegroup name	P 1 21 1
Unit cell lengths	58.177, 60.816, 87.021
Unit cell angles	90.00, 108.05, 90.00

Refinement procedure

Resolution	40.560 - 2.000
R-factor	0.2134
Rwork	0.211
R-free	0.25930
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	REFMAC

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.000
Number of reflections	33549

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.4	295	20% PEG 3350, 0.2 M sodium sulfate, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 295K