3RSM
Crystal structure of S108C mutant of PMM/PGM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Collection date | 2010-05-17 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 71.034, 72.806, 92.997 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.840 - 2.100 |
| R-factor | 0.21463 |
| Rwork | 0.213 |
| R-free | 0.25252 |
| Structure solution method | refinement |
| Starting model (for MR) | 1k35 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.387 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.843 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.060 | 0.513 |
| Number of reflections | 29767 | |
| <I/σ(I)> | 11 | 2.3 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 4.8 | 4.72 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 1.4 Na,K tartrate, 100 mM Na Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
