3RPG

Bmi1/Ring1b-UbcH5c complex structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	298
Detector technology	CCD
Collection date	2010-02-23
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.541
Spacegroup name	P 32 2 1
Unit cell lengths	107.890, 107.890, 77.572
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.822 - 2.648
R-factor	0.2186
Rwork	0.217
R-free	0.24310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2H0D AND 2FUH
RMSD bond length	0.004
RMSD bond angle	0.900
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.6.4_486))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.740
High resolution limit [Å]	2.648	2.648
Rmerge	0.100	0.459
Number of reflections	15512
Completeness [%]	100.0	100
Redundancy	10.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	277	40% MPD, 0.1 M MES pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K