3RP7
Crystal Structure of Klebsiella pneumoniae HpxO complexed with FAD and uric acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-18 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.023, 67.840, 82.236 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.920 - 2.042 |
| R-factor | 0.194 |
| Rwork | 0.192 |
| R-free | 0.23180 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.035 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | REFMAC |
| Refinement software | PHENIX (1.7_650) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.110 |
| High resolution limit [Å] | 2.040 | 4.390 | 2.040 |
| Rmerge | 0.093 | 0.068 | 0.311 |
| Number of reflections | 24401 | ||
| <I/σ(I)> | 22.8 | ||
| Completeness [%] | 99.7 | 98.9 | 99.3 |
| Redundancy | 5.5 | 5 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop | 291 | 0.05 M KH2PO4, 20% PEG 8000, hanging drop, temperature 291K |
