3RMX
Crystal structure of HCR/D F1240A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-18 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 94.570, 115.630, 107.240 |
| Unit cell angles | 90.00, 91.91, 90.00 |
Refinement procedure
| Resolution | 36.610 - 2.750 |
| R-factor | 0.239 |
| Rwork | 0.239 |
| R-free | 0.29300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3n7j |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MERLOT |
| Refinement software | CNS (1.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.610 | 2.800 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Number of reflections | 59660 | |
| <I/σ(I)> | 14.7 | 2.7 |
| Completeness [%] | 99.0 | 90.2 |
| Redundancy | 4 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 292 | 0.1 M MOPS pH 7.0, 10% PEG4K, 12.5% MPD, 100 mM NaCl, VAPOR DIFFUSION, temperature 292K |
