3RJ3
Complement components factor H CCP19-20 (S1191L mutant) and C3D in complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-29 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 74.750, 82.620, 85.400 |
| Unit cell angles | 112.61, 110.26, 99.84 |
Refinement procedure
| Resolution | 41.604 - 2.350 |
| R-factor | 0.1926 |
| Rwork | 0.191 |
| R-free | 0.23160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3oxu |
| RMSD bond length | 0.011 |
| RMSD bond angle | 0.733 |
| Refinement software | PHENIX ((phenix.refine: 1.6.2_432)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.470 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.075 | 0.597 |
| Number of reflections | 69803 | |
| <I/σ(I)> | 11.6 | 2.2 |
| Completeness [%] | 94.8 | 83.6 |
| Redundancy | 3.9 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 290 | 4% w/v PEG 8000, 0.1 M Tris HCl, pH 8.8, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
