3RIA
C. elegans glutamate-gated chloride channel (GluCl) in complex with Fab, ivermectin and iodide.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.490 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 155.099, 155.099, 578.257 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.775 - 3.800 |
| R-factor | 0.2671 |
| Rwork | 0.266 |
| R-free | 0.28030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3rhw |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.728 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.940 |
| High resolution limit [Å] | 3.800 | 3.800 |
| Rmerge | 0.142 | 0.672 |
| Number of reflections | 69600 | |
| <I/σ(I)> | 11.6 | 2.7 |
| Completeness [%] | 98.3 | 97.7 |
| Redundancy | 4.9 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 277 | 21-23% PEG 400, 50 mM sodium citrate pH 4.5, 70 mM sodium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
