3RH5
DNA Polymerase Beta Mutant (Y271) with a dideoxy-terminated primer with an incoming deoxynucleotide (dCTP)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-02 |
| Detector | RIGAKU SATURN 92 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.220, 79.920, 55.090 |
| Unit cell angles | 90.00, 107.93, 90.00 |
Refinement procedure
| Resolution | 20.705 - 2.096 |
| R-factor | 0.2155 |
| Rwork | 0.208 |
| R-free | 0.28090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fms |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.202 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.705 | |
| High resolution limit [Å] | 2.096 | 2.100 |
| Rmerge | 0.111 | 0.567 |
| Number of reflections | 24357 | |
| <I/σ(I)> | 10 | 2.25 |
| Completeness [%] | 98.1 | 97.4 |
| Redundancy | 3.5 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 16% PEG3350, 350 mM sodium acetate, 50 mM imidazole, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
