3RFS
Design of a binding scaffold based on variable lymphocyte receptors of jawless vertebrates by module engineering
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-14 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.733, 107.693, 71.277 |
| Unit cell angles | 90.00, 91.06, 90.00 |
Refinement procedure
| Resolution | 33.830 - 1.700 |
| R-factor | 0.19911 |
| Rwork | 0.197 |
| R-free | 0.24206 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.097 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.830 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 49492 | |
| Completeness [%] | 99.9 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 293 | 0.1M Tris-HCl, 30%(w/v) polyethylene glycol 4000, 0.2M magnesium chloride hexahydrate, pH 8.5, VAPOR DIFFUSION, temperature 293K |
