3REP
Crystal structure of the ILK/alpha-parvin core complex (MnATP)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-01-22 |
Detector | RIGAKU SATURN 944+ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 44.022, 116.280, 47.284 |
Unit cell angles | 90.00, 101.53, 90.00 |
Refinement procedure
Resolution | 34.640 - 1.800 |
R-factor | 0.20583 |
Rwork | 0.204 |
R-free | 0.23783 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 3kmw |
RMSD bond length | 0.017 |
RMSD bond angle | 1.472 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | CNS |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.130 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.034 | 0.136 |
Number of reflections | 40605 | |
Completeness [%] | 94.2 | 76.2 |
Redundancy | 5.15 | 4.22 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 277 | 50 mM Bis-Tris Propane, 12.5 (w/v) PEG 5000 MME, 5% (v/v) 1-propyl alcohol, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |