3REP
Crystal structure of the ILK/alpha-parvin core complex (MnATP)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-01-22 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.022, 116.280, 47.284 |
| Unit cell angles | 90.00, 101.53, 90.00 |
Refinement procedure
| Resolution | 34.640 - 1.800 |
| R-factor | 0.20583 |
| Rwork | 0.204 |
| R-free | 0.23783 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3kmw |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.472 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.130 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.034 | 0.136 |
| Number of reflections | 40605 | |
| Completeness [%] | 94.2 | 76.2 |
| Redundancy | 5.15 | 4.22 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 277 | 50 mM Bis-Tris Propane, 12.5 (w/v) PEG 5000 MME, 5% (v/v) 1-propyl alcohol, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
