3RDO

Crystal structure of R7-2 streptavidin complexed with biotin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2008-07-10
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9791
Spacegroup name	I 41 2 2
Unit cell lengths	57.360, 57.360, 184.067
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.260 - 1.404
R-factor	0.15182
Rwork	0.151
R-free	0.17094
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3rdm
RMSD bond length	0.008
RMSD bond angle	1.390
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER (2.1.2)
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.450
High resolution limit [Å]	1.400	3.020	1.400
Rmerge	0.048	0.027	0.488
Number of reflections	29759
<I/σ(I)>	16.7
Completeness [%]	96.3	99.2	70.1
Redundancy	5.8	6.3	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	0.1 M ammonium sulfate, 0.1 M Tris, pH 7.5, 20% PEG 1500, VAPOR DIFFUSION, SITTING DROP, temperature 298K