3RBU
N-terminally AviTEV-tagged Human Glutamate Carboxypeptidase II in complex with 2-PMPA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-24 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 101.247, 130.559, 158.897 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.460 - 1.600 |
| R-factor | 0.15774 |
| Rwork | 0.157 |
| R-free | 0.18233 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2pvw |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.689 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | REFMAC |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.081 | 0.517 |
| Number of reflections | 136479 | |
| <I/σ(I)> | 19.8 | 2.4 |
| Completeness [%] | 99.0 | 91.9 |
| Redundancy | 7 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 33% pentaerythritol propoxylate, 1% PEG3350, 100 mM Tris-HCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
