3RB6

Dpo4 extension ternary complex with 3'-terminal primer A base opposite the 3-methylcytosine (m3c) lesion

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2006-07-20
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97949
Spacegroup name	P 1 21 1
Unit cell lengths	52.915, 109.328, 100.828
Unit cell angles	90.00, 101.07, 90.00

Refinement procedure

Resolution	20.000 - 2.700
R-factor	0.205
Rwork	0.203
R-free	0.24722
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3rb4
RMSD bond length	0.008
RMSD bond angle	1.438
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AMoRE
Refinement software	REFMAC (5.6.0107)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.790
High resolution limit [Å]	2.700	2.700
Number of reflections	30903
Completeness [%]	98.3	95.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	100 mM HEPES, pH 7.0, 100 mM calcium acetate and 10% PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 293K