3RAN

CANINE GDP-RAN Q69L MUTANT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 5.2R
Synchrotron site	ELETTRA
Beamline	5.2R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-01-29
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	63.090, 59.170, 116.370
Unit cell angles	90.00, 100.68, 90.00

Refinement procedure

Resolution	6.000 - 2.300 *
Rwork	0.176
R-free	0.27100 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	EBI-1279
RMSD bond length	0.012 *
RMSD bond angle	2.100 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.300	2.420
High resolution limit [Å]	2.300	2.300
Rmerge	0.035	0.155
Total number of observations	76123 *
Number of reflections	35632
<I/σ(I)>	14.8	4.9
Completeness [%]	94.7	94.9
Redundancy	2.1	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.4 *	18 *	SEE PUBLICATION, pH 7.2

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	PEG1000	35 (%)
3	1	reservoir		20 (mM)
4	1	reservoir	Tris-HCl	50 (mM)