3R3L
Structure of NP protein from Lassa AV strain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-07 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97745 |
| Spacegroup name | P 3 |
| Unit cell lengths | 176.320, 176.320, 56.580 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.449 |
| R-factor | 0.18548 |
| Rwork | 0.184 |
| R-free | 0.21908 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mwp |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.960 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 88.160 |
| High resolution limit [Å] | 2.440 |
| Number of reflections | 37576 |
| Completeness [%] | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | Protein at 7.5 mg/ml. Reservoir: 7% PEG300, 0.5 M KCl, 10% glycerol, VAPOR DIFFUSION, HANGING DROP |
