3R3I
Crystal Structure of C-terminal truncation of UDP-glucose Pyrophosphorylase of Homo sapiens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-31 |
| Detector | MAR |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 140.266, 140.266, 315.284 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.980 - 3.570 |
| R-factor | 0.2353 |
| Rwork | 0.233 |
| R-free | 0.28620 |
| Starting model (for MR) | 3r2w |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.559 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.981 | 3.610 |
| High resolution limit [Å] | 3.566 | 3.566 |
| Rmerge | 0.095 | 0.649 |
| Number of reflections | 42839 | |
| Completeness [%] | 97.9 | 61.2 |
| Redundancy | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 290 | magnesium sulfate, PEG3350, glycerol, HEPES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
