3R2V

Crystal structure of polymerase basic protein 2 E538-R753 from Influenza A virus A/Yokohama/2017/03 H3N2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-03-04
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97946
Spacegroup name	P 21 21 2
Unit cell lengths	52.810, 106.540, 33.490
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.300
R-factor	0.1816
Rwork	0.180
R-free	0.20940
Structure solution method	SAD, MOLECULAR REPLACEMENT
Starting model (for MR)	2vy6
RMSD bond length	0.009
RMSD bond angle	1.278
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.1.4)
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.330
High resolution limit [Å]	1.300	1.300
Rmerge	0.040	0.319
Number of reflections	46293	2983
<I/σ(I)>	21.66	3.27
Completeness [%]	97.6	86.7
Redundancy	5.4	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	289	InvaB.07055.c.D17 PD00118 at 24.51 mg/mL against 0.1 M MIB pH 6.0, 25% PEG 1500, 100 mM NaI, crystal tracking ID 220930b6, VAPOR DIFFUSION, SITTING DROP, temperature 289K