3QQC
Crystal structure of archaeal Spt4/5 bound to the RNAP clamp domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-10-18 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.2783 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 168.570, 107.040, 51.090 |
| Unit cell angles | 90.00, 97.10, 90.00 |
Refinement procedure
| Resolution | 50.700 - 3.300 |
| R-factor | 0.195 |
| Rwork | 0.193 |
| R-free | 0.24700 |
| Structure solution method | Molecular Replacement with SAD |
| Starting model (for MR) | 1y1w |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.370 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.9.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.700 | 3.480 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Number of reflections | 13603 | |
| <I/σ(I)> | 7.4 | 2.1 |
| Completeness [%] | 99.8 | 99.6 |
| Redundancy | 5.7 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 293 | 10% PEG 8000, 0.1 M sodium/potassium phosphate, 0.15 M guanidinium hydrochloride, 0.2 M sodium chloride, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
