3QPW
PFKFB3 in complex with Aluminum Tetrafluoride
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CAMD BEAMLINE GCPCC |
| Synchrotron site | CAMD |
| Beamline | GCPCC |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-26 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.3808 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 102.709, 102.709, 257.044 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.250 |
| R-factor | 0.20162 |
| Rwork | 0.200 |
| R-free | 0.24216 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.814 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | REFMAC (5.2.0019) |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.330 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Number of reflections | 38530 | |
| Completeness [%] | 99.5 | 98.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 300 | 00 mM Tris-HCl, pH 7.5, 20-25% ethylene glycol, 200-400mM Tartaric Acid, 5% glycerol,12% polyethylene glycol 4000, VAPOR DIFFUSION, SITTING DROP, temperature 300K |
