3QLN

Crystal structure of ATRX ADD domain in free state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2010-04-06
Detector	ADSC QUANTUM 315
Wavelength(s)	1.2827
Spacegroup name	P 32
Unit cell lengths	74.167, 74.167, 54.091
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.115 - 1.901
R-factor	0.1298
Rwork	0.127
R-free	0.15830
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	0.919
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	PHENIX (1.6.2_432)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.900
Rmerge	0.063
Number of reflections	52327
<I/σ(I)>	43.6
Completeness [%]	99.9
Redundancy	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	25% (v/v) PEG 4000, 100mM HEPES-NaOH, 0.2M KCL, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K