3QK1

Crystal Structure of Enterokinase-like Trypsin Variant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2006-10-05
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	60.710, 63.760, 69.690
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.190 - 2.080
R-factor	0.1875
Rwork	0.182
R-free	0.23660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1mts
RMSD bond length	0.023
RMSD bond angle	1.931
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall
Low resolution limit [Å]	31.190
High resolution limit [Å]	2.080
Rmerge	0.068
Number of reflections	16758
<I/σ(I)>	7.41
Completeness [%]	99.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	0.1 M imidazole 0.2 M ammonium sulfate 30% (w/v) PEG 8000, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K