3QID
Crystal structures and functional analysis of murine norovirus RNA-dependent RNA polymerase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Detector technology | CCD |
| Collection date | 2009-04-14 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.00 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 120.557, 196.262, 109.339 |
| Unit cell angles | 90.00, 114.23, 90.00 |
Refinement procedure
| Resolution | 49.070 - 2.500 |
| R-factor | 0.2016 |
| Rwork | 0.199 |
| R-free | 0.24490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sh2 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.247 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.096 | 0.096 |
| Number of reflections | 78636 | |
| <I/σ(I)> | 21.5 | 21.5 |
| Completeness [%] | 99.2 | 99.2 |
| Redundancy | 7.6 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 1.26M (NH4)2SO4, 100mM cacodylate , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
