3QEJ

S74E-dCK mutant in complex with UDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	93
Detector technology	IMAGE PLATE
Collection date	2010-06-17
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	P 41
Unit cell lengths	68.430, 68.430, 120.370
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.490
R-factor	0.20433
Rwork	0.201
R-free	0.23621
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.387
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0053)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.500
Number of reflections	18394
Completeness [%]	95.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		298	15 mg/ml protein mixed with 0.9-1.5M trisodium citrate di-hydrate and 100 mM Tris, pH 7.5, VAPOR DIFFUSION, temperature 298K