3QEB

Crystal structure of human exonuclease 1 Exo1 (WT) in complex with DNA and Mn2+ (complex III)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2010-07-10
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.00
Spacegroup name	P 43 21 2
Unit cell lengths	70.406, 70.406, 182.883
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.030 - 3.000
R-factor	0.2258
Rwork	0.224
R-free	0.26830
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.001
RMSD bond angle	0.412
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.6.2_432))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.100
High resolution limit [Å]	3.000	3.000
Number of reflections	11100
Completeness [%]	83.3	53.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	290	PEG 6K, MES, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 290K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	PEG 6K
2	1	1	MES
3	1	2	PEG 6K
4	1	2	MES