3QBT
Crystal structure of OCRL1 540-678 in complex with Rab8a:GppNHp
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-09-18 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 157.147, 55.340, 173.836 |
| Unit cell angles | 90.00, 91.93, 90.00 |
Refinement procedure
| Resolution | 47.378 - 2.000 |
| R-factor | 0.2096 |
| Rwork | 0.208 |
| R-free | 0.24110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | codes 1r2q 2qv2 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.412 |
| Data scaling software | XSCALE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.378 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.055 | 0.334 |
| Number of reflections | 99857 | |
| <I/σ(I)> | 4.2 | |
| Completeness [%] | 98.3 | 97 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 20% (w/v) PEG 4000, 20% (v/v) glycerol, 0.16 M ammonium sulphate, 0.1 M sodium acetate, pH 4.6, vapor diffusion, hanging drop, temperature 293K |
