3Q7J

Engineered Thermoplasma Acidophilum F3 factor mimics human aminopeptidase N (APN) as a target for anticancer drug development

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2009-10-19
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9792
Spacegroup name	P 21 21 2
Unit cell lengths	114.717, 183.101, 105.154
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.953 - 2.910
R-factor	0.2059
Rwork	0.202
R-free	0.27130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1z5h
RMSD bond length	0.009
RMSD bond angle	1.228
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.6.2_432))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.000
High resolution limit [Å]	2.900	6.240	2.900
Rmerge	0.128	0.059	0.529
Number of reflections	49142
<I/σ(I)>	16.14	30.32	2.68
Completeness [%]	99.9	99.9	99.4
Redundancy	7.2	7	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	18% PEG 2000, 0.2M LITHIUM SULPHATE, 0.1M Tris-HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K