3Q1S
HIV-1 neutralizing antibody Z13e1 in complex with epitope display protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-25 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.058 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.623, 99.216, 105.533 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.630 - 2.150 |
| R-factor | 0.20984 |
| Rwork | 0.207 |
| R-free | 0.26024 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fn0 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.701 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 49.630 |
| High resolution limit [Å] | 2.150 |
| Number of reflections | 33242 |
| <I/σ(I)> | 16 |
| Completeness [%] | 99.9 |
| Redundancy | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 19% PEG 4000, 10% isopropanol, 0.1M hepes, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
