3Q1R
Crystal structure of a bacterial RNase P holoenzyme in complex with TRNA and in the presence of 5' leader
Replaces: 3OKBExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-02-10 |
Detector | RAYONIX MX-225 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 169.920, 169.920, 185.530 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 4.390 |
High resolution limit [Å] | 4.200 | 4.200 |
Rmerge | 0.081 | 0.463 |
Number of reflections | 17674 | |
<I/σ(I)> | 10.7 | 2.4 |
Completeness [%] | 76.4 | 7.8 |
Redundancy | 5.7 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 303 | 1.8M LiSO4, 0.5M sodium cacolydate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 303K |